Figure 8: Pharmacological inhibition of P110δ leads to spontaneous corneal lymphangiogenesis.
(a) Morphometric comparison of corneal lymph vascularized areas in WT mice treated with or without intraperitoneal injection of IC87118 (30 mg kg−1) and JSH23 (10 mg kg−1). Scale bars, 200 μm. (b) Quantification of percentage in vascularized areas over the whole corneas shown in a (***P<0.001, **P<0.01, *P<0.05, n=4, two-tailed t-test). (c) qPCR analyses of Vegf-c transcripts in corneas from WT and Mmp14−/− mice treated with or without IC87118 (30 mg kg−1) and JSH23 (10 mg kg−1) (**P<0.01, *P<0.05, n=4, two-tailed t-test). The experiments were repeated at least three times. (d) Whole-mounted corneal immunostaining using VEGFR-3 (red) and CD11b (green) antibodies in P10 WT and Mmp14−/− mice with or without the treatment of IC87118 and JSH23 (n=4). Scale bars, 25 μm Data represent the mean±s.e.m.
