Figure 3: Eaf2−/− mice have enlarged GC with decreased apoptosis compared with WT mice. | Nature Communications

Figure 3: Eaf2−/− mice have enlarged GC with decreased apoptosis compared with WT mice.

From: EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity

Figure 3

GC formation in the spleen on day 14 after primary immunization with NP–CGG. (a) Left, representative PNA staining (blue) of spleen sections in WT and Eaf2−/− mice. Right, average size of GC in each mouse. Over 20 GC were measured in each mouse and the average size was calculated. Each symbol represents the average GC size in one mouse. The results of three pairs of WT and Eaf2−/− mice are shown. (b) Left, representative TUNEL staining (brown spots indicated by arrows are TUNEL+), Right, TUNEL+ spot numbers per each GC. Brown TUNEL+ spots in 7–10 GC of each mouse were counted. The results of two pairs of WT and Eaf2−/− mice are shown. For both a and b, original magnification: × 200. Bars, 50 μm. (c) EdU incorporation by B220+PNA+ GC B cells in WT and Eaf2−/− mice. Left, representative FACS profiles of EdU levels; right, results of 5 WT and 6 Eaf2−/− mice. (d) EdU incorporation by B220+PNA− non-GC B cells in WT and Eaf2−/− mice. The red bar indicates the mean value of each group. **P<0.01 (unpaired t-test).

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