Figure 6: BRD3R regulates mitosis during reprogramming.
From: The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis
(a) BRD3R increases cell population in M phases on day 6 of reprogramming (mean±s.d., n=3; *P<0.05; **P<0.01; t test). (b) Representative flow cytometry histograms from experiments in (a). Cell cycle analysis was performed using Dean–Jett–Fox model on FlowJo. (c) BRD3R increases numbers of mitotic cells as revealed in shake-off experiments of reprogramming cells on day 4 of reprogramming (mean±s.d., n=3; *P<0.05; t-test). (d) BRD3R decreases senescence cells during reprogramming. Shown are percentages of SA-β-galactosidase+ cells (on day 5 of reprogramming; n=10; **P<0.01; ***P<0.01; t-test). (e) Representative staining images for SA-β-galactosidase (blue). Red star indicates cells with morphology resembling those of mitotic cells. Scale bar, 50 μm. (f) BRD3R associates with mitotic chromatin at all mitotic phases (day 3 of reprogramming, visualized using HA antibody for HA-tagged BRD3R). Different stages of mitotic phases are indicated. (g) Pol II dissociates from mitotic chromatin. Scale bars in f and g, 10 μm.
