Figure 4: IL-15koRAG2ko mice with colitis have diminished percentage and altered phenotype of Treg.
From: IL-15-dependent balance between Foxp3 and RORγt expression impacts inflammatory bowel disease
(a–e) About 2 × 105 CD4+ T lymphocytes from B6 mice were transferred into RAG2ko or IL-15koRAG2ko mice killed 4 weeks later. (a) Expression of CD25 and Foxp3 on CD4+ T cells in the injected IL-15koRAG2ko and RAG2ko mice. (b) Frequency among live CD4+ T cells of CD4+CD25+Foxp3+ T cells (Treg) recovered from adoptively transferred IL-15koRAG2ko and RAG2ko mice. (c) Expression of Treg-related markers: Helios and CD103 by Treg cells isolated from the colon of IL-15koRAG2ko (black line) and from RAG2ko recipient mice (grey shadow). (d) Frequency among Treg (CD4+CD25+Foxp3+) of Helios- (upper panel) and CD103 (lower panel) -expressing cells recovered from the injected IL-15koRAG2ko and RAG2ko mice. (b–d) Open circles RAG2ko mice; black circles: IL-15koRAG2ko mice. (e) Expression of CD25 and Foxp3 on CD4+ T cells in the colon of unmanipulated IL-15ko or B6 mice (upper panel) and frequency among live CD4+ T cells of CD4+CD25+Foxp3+ Treg in the colon, the ILN and the spleen of unmanipulated IL-15ko or B6 mice (lower panel). Data compiled from two separate experiments with at least three mice per group. Each symbol represents an individual mouse. All graphs indicate means; error bars denote s.e.m.; Student’s t-test values: *P<0.05; ***P<0.001.
