Figure 7: CD4+ T cells with impaired IL-15 signaling respond to TCR stimulation by down-regulation of pSTAT5 and up-regulation of Runx1.
From: IL-15-dependent balance between Foxp3 and RORγt expression impacts inflammatory bowel disease
(a) IL-15ko, IL-15Rαko (IL-15 receptor alpha deficient) and B6 mice received i.p. 50 μg α-CD3 Ab and were killed 2 h later. (b) Expression of p-STAT5 (phosphorylated signal transducer and activator of transcription 5) on CD4+ T cells in the LN sampled from the injected mice and re-stimulated 20 min in vitro with recombinant mouse (rm) IL-15. (c) Median fluorescence intensity (MFI) of p-STAT5 expression on CD4+ T cells isolated from the injected mice and re-stimulated 20 min in vitro with rmIL-15. (d) Expression of Runx1 (runt-related transcription factor-1) in CD4+ T cells isolated from the LN of IL-15ko, IL-15Rαko and B6 mice 2 h after injection of α-CD3 Ab. (e) MFI of Runx1 expression on CD4+ T cells isolated from the injected mice. Black line: IL-15ko, black shadow: IL-15Rαko, grey shadow: B6. Black circles: IL-15ko, black diamonds: IL-15Rαko, open circles B6. (b–e) Data compiled from two separate experiments with at least two mice per group. Each symbol represents an individual mouse. One-way analysis of variance (P<0.01), Tukey’s post-test values: *P<0.05; **0.01<P<0.05.
