Figure 1: Differential DNA methylation of DGKA and fibrosis.

Distribution of differential methylation (a) and genomic site distribution (b) in a representative set of differentially methylated CpG sites (P value <0.05 after beta regression; n=177) from Illumina 450 K array data according to the adjusted P values. (c) A heat map of Illumina 450 K DNA methylation values at differentially methylated sites (n=35, P<0.05 (adjusted Wald test) estimated methylation difference >10%) separating fibrosis (orange) and non-fibrosis (green) samples. Rows show gene names or CpG site IDs when gene annotation is not available. Scale bar indicates DNA methylation (in %). (d) Differential methylation at the DGKA locus measured by EpiTYPER technology in a total sample set of 75 patient-derived fibroblasts. P value was determined using Wilcoxon rank-sum testing. Bars indicate median. (e) Detailed interrogation of DNA methylation using EpiTYPER in two distinct patient fibroblasts (fibrosis and fibrosis-free) at the DGKA 5′ UTR (upper panel) and the differentially methylated region (lower panel). Each dot represents an individual CpG per patient from EpiTYPER analysis. The genomic DMR region interrogated (grey), CpG islands (green) and DGKA transcripts (blue) are shown. Intergenic, no gene context assigned; body, gene body/intragenic localization; TSS200/1,500: 200 bp/1,500 bp window around transcription start site; UTR, untranslated region; 1st exon, localization within the first exon of a known transcript.