Figure 1: GPCR profiling and Gq signalling in murine brown adipocytes.
From: The Gq signalling pathway inhibits brown and beige adipose tissue
(a) Venn diagram showing unique and overlapping GPCRs expressed in murine brown preadipocytes and mature murine BA. (b) Pie chart of GPCR-linkage classification in BA. (c) Pie chart of GPCR-linkage classification in brown preadipocytes. (d) Oil Red O stain of BA chronically treated with and without FR and after knockdown of Gq with shRNA (shGq); Ctrl, control. Scale bar, 1 cm. (e–h) Representative immunoblots (e,g) and quantification (f,h) of adipogenic markers (aP2; PPARγ) and thermogenic marker (UCP1) of BA after indicated treatment. ANOVA, *P<0.05. (i,j) Representative traces of label-free DMR (i) and concentration-effect curve (j) in cells stably expressing Gq DREADD (Dq) treated with indicated concentrations of CNO. (k,l) Representative immunoblots (k) and quantification (l) of aP2, PPARγ and UCP1 in BA transduced with control virus (LVctrl) or virus containing Dq and differentiated in presence or absence of CNO. ANOVA, *P<0.05. (m,n) Representative immunoblots (m) and quantification (n) of aP2, PPARγ and UCP1 in BA transduced with control virus (LVGFP) or virus expressing constitutively active Gq (LVGqQL). (a–c), n=3 independent arrays. (d–h) n=4. (i,j) n=3. (k-l) n=4. (m,n) n=5. ANOVA, *P<0.05. All data are shown as mean±s.e.m.
