Table 1 Growth phenotypes and status of autolysis and catalysis of mutants.
From: A unified mechanism for proteolysis and autocatalytic activation in the 20S proteasome
Mutant | Viability | Temperature sensitivity | Autolysis state of the mutant subunit* | Activity of the mutant subunit |
|---|---|---|---|---|
WT | ++++ | + | + | +++ |
β1-T1A (ref. 13) | ++++ | + | − | − |
β2-T1A (ref. 13) | +++ | ++ | − | − |
β1-T1A β2-T1A (ref. 13) | +++ | ++ | − | − |
β5-T1A | +/− | ++++ | − | − |
β5-H(-2)A-T1A | +/− | ND | − | − |
β5-H(-2)T-T1A | +/− | ND | − | − |
β5-H(-2)L-T1A | ++ | ND | − | − |
β5-T1A, pp trans, nat1Δ | +/− | ++++ | pp trans | − |
β5-T1A-K81R | + | ++++ | − | − |
β5-H(-2)A-T1A-K81R | +/− | ND | − | − |
β5-H(-2)T-T1A-K81R | +/− | ND | − | − |
β5-H(-2)L-T1A-K81R | ++ | ND | − | − |
β5-H(-2)A | ++++ | ++ | + | +++ |
β5-H(-2)K | ++++ | ++ | + | +++ |
β5-H(-2)F | ++++ | +++ | + | ++ |
β5-H(-2)N | ++++ | +++ | + | ++ |
β5pp-β1 (ref. 18) | ++++ | + | +/− | +/− |
β2-T(-2)V | ++++ | + | − | − |
β5-L(-49S)-K33A (ref. 13) | + | ++++ | − | − |
β5-K33A, pp trans13 | + | ++++ | pp trans | +/− |
β5-F(-45)S-K33R (ref. 13) | ++ | ++++ | + | − |
β5-D17N | +/− | ++++ | ND† | ND† |
β5-L(-49)S-D17N | + | ++++ | +/− | +/− |
β5-D17N, pp trans | + | ++++ | pp trans | +/− |
β5-D166N | ++ | ++++ | + | +/− |
β5-D166N, pp trans | +++ | ++++ | pp trans | +/− |
β5-T1S | +++ | ++++ | + | ++ |
β5-T1C | ++ | ++++ | + | − |
