Figure 5: Aberrant pubertal gene expression in CKO is partially rescued in DKO. | Nature Communications

Figure 5: Aberrant pubertal gene expression in CKO is partially rescued in DKO.

From: Genetic suppression reveals DNA repair-independent antagonism between BRCA1 and COBRA1 in mammary gland development

Figure 5

(a) Heatmap illustrates the gene expression changes in total mammary epithelial cells of CKO and DKO as compared with their corresponding WT littermates (n=3) at three time points (4, 6 and 8 wks). The gene expression levels in WT are set as 1. (b) Expression patterns for two representative pubertal genes that are affected by CKO and partially rescued by DKO. The lowest expression level in each graph is set at 1. (c) Confirmation of the microarray data by gene-specific RT-PCR for a number of pubertal genes, using RNA samples from 6-wk old mice. The result is average of values from 3 animals in each genotype. β-Actin mRNA was used for normalization. *P<0.05 by Student’s t-test. Statistical analysis was conducted between WT and CKO, and between CKO and DKO. Error bars represent s.e.m. wk, week.

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