Figure 3: Bcl-2 is dispensable for ISCs during regeneration.
From: Bcl-2 is a critical mediator of intestinal transformation
(a) Panel of sections stained for cleaved caspase-3 (CC-3) in Lgr5.Bcl-2+/+ mice (top) and Lgr5.Bcl-2fl/fl mice (bottom) 5 weeks after tamoxifen induction and 4 h after 6 Gy full-body irradiation. Scale bars, 100 μm. (b) Graph displaying the percentage of CC-3 foci per crypt for Lgr5.Bcl-2+/+ and Lgr5.Bcl-2fl/fl mice at the indicated time points post 6 Gy irradiation. A minimum of 80 crypts was counted for each genotype and time point. Error bars represent the s.e.m., *P<0.05, ****P<0.0001, Student’s t-test. (c) Graph showing the percentage of Lgr5-GFP+ cells in the small intestine for non-irradiated controls and post-irradiated mice at the indicated days (n=3 mice per condition, per time point). Analyses are performed by FACS. Error bars represent the s.e.m., NS, not significant, *P<0.05, Student’s t-test.
