Table 1 Proteins complexes produced using the strategy described.
From: Production of unstable proteins through the formation of stable core complexes
Protein | Stabilized by | Strategy | References | |
|---|---|---|---|---|
Human steroid nuclear receptors | ERα-E | Ligand (oestrogen) | Ligand in cell culture | |
Thioredoxin fusion | Keep Thioredoxin (TRX) fusion for crystallization | |||
3Cys to Ser mutations | Mutations mimic the conformational changes induced by the ligand | |||
ERβ-EF | TIF2 (623–772) | In vitro reconstitution (co-cell lysis) | This article | |
GRtm-E | TIF2 (623–772) and solubility mutant | In cell co-expression | This article | |
HIV-1 pre-integration complexes | IN | hLEDGF | In vitro reconstitution (dialysis) | |
hLEDGF/hINI1(174–289) | ||||
hLEDGF | Expression in E. coli, insect and mammalian cells and in vitro reconstitution | This article |
