Figure 2: Effects of Sik3 deletion on osteoarthritis development in DMM-operated knees.
From: Pterosin B prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting Sik3
(a) Protocol of experiments: 7-week-old mice were intraperitoneally injected with tamoxifen for 5 consecutive days, had their right knees subjected to DMM or sham surgeries, and were killed at 16 weeks of age. (b) Histology of proximal tibia in sham-operated knees. Safranin O-fast green-iron haematoxylin staining. The thickness of articular cartilage and growth plate cartilage are shown in the graph. t-test. (c) Histology of articular cartilage of proximal tibia in DMM-operated knees. Boxed regions are shown in the bottom panels. OARSI scores of articular cartilage in sham- and DMM-operated knees are shown. Two blinded scorers gave consistent results. Mann–Whitney test. (d) Immunohistochemistry for Col10 in knees. Boxed regions in the top panels are shown in the bottom panels. The percentage of lacunae surrounded by Col10-positive matrix per total number of lacunae are shown in the right graph. Tukey–Kramer post hoc test. Error bars denote means±s.d. Numbers of mice examined are indicated at the bottom of the graphs. *P<0.05 and **P<0.01. NS, not significantly different. Scale bars, 100 μm.
