Figure 5: Effects of pterosin B on human articular chondrocytes and hiPSC-derived chondrocytes.
From: Pterosin B prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting Sik3
(a) Semiserial histological sections of cultured pellets of human articular cartilage in the presence or absence of 300 μM pterosin B were stained with safranin O-fast green-iron haematoxylin and von Kossa and immunostained for COL10. Boxed regions in the left panels are shown in the right. Area of von Kossa-positive regions per total area of pellets are shown; n=3 pellets. (b) Real-time RT–PCR expression analysis of marker genes in pellet culture of human articular chondrocytes in the presence or absence of 300 μM pterosin B; n=3 pellets. (c) Semiserial histological sections of hiPSC-derived cartilage particles treated with or without 300 μM pterosin B were stained with safranin O-fast green-iron haematoxylin and von Kossa and immunostained for COL2 and COL10. Boxed regions in the left panels are shown in the right. The images are representative of three pellets each. (d) Real-time RT–PCR expression analysis of marker genes in hiPSC-derived cartilage particles in the presence or absence of 300 μM pterosin B; n=3 pellets. Error bars denote means±s.d. *P<0.05, **P<0.01 and NS, not significantly different by the t-test. Scale bars, 100 μm.
