Figure 6: Cyclic optical activation of DGH GABAergic interneurons has a long-lasting inhibitory effect on seizures.
From: Optogenetic dissection of ictal propagation in the hippocampal–entorhinal cortex structures
(a,b) Representative examples of spectrograms of LFPs in DGH of non-stimulated (a) and optically stimulated (b) mice after KA injection. Data were normalized to the maximal log10(power) value across the whole 0–80 Hz frequency interval. Each blue arrow represents an optical stimulation cycle (473 nm, 5 ms pulse duration at 130 Hz, 1 min on, 5 min off); a total of 16 cycles were delivered within a 90-min period. Power values were normalized to the total power in the pre-KA period. (c) Power quantification of LFPs in the DGH of non-stimulated (n=7 mice) and optically stimulated (n=8 mice) group after kainate. (d) The seizure numbers for Racine grade IV–V behaviours calculated in each 30-min segment during a 120-min period after KA injection. (e) Normalized seizure numbers in the stimulated (n=7 mice) and non-stimulated (n=7 mice) groups. The shaded area shows the 90 min cyclic stimulation period. Error bars represent s.e.m. Stars represent significant differences (*P<0.05, **P<0.01, ***P<0.001, paired t-test).
