Figure 8: Loss of MYO7A sensitizes cells to cytokine-induced cell death in a CASP8-dependent manner. | Nature Communications

Figure 8: Loss of MYO7A sensitizes cells to cytokine-induced cell death in a CASP8-dependent manner.

From: The unconventional myosin CRINKLED and its mammalian orthologue MYO7A regulate caspases in their signalling roles

Figure 8

(a) WB analysis of activated CASP8 (p43 and p18 cleavage products), MYO7A, RIPK1 or ACTIN following the indicated treatments in NIH-3T3 cells. Depicted are representative westernblot images of three biological repeats. (b) DEVDase assays from lysates of NIH-3T3 cells subjected to siRNA targeting Myo7A or Casp8, and exposed to the indicated treatments. (c) FACS analysis of PI-positive NIH-3T3 or SWISS-3T3 cells subject to siRNA knockdown of the indicated genes followed by the indicated treatments. Error bars represent s.d. P-values are indicated. Experiments were conducted in triplicates.

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