Figure 4: SR-SIM measurement of an LSEC membrane stain obtained on a GE Healthcare DeltaVision|OMX. | Nature Communications

Figure 4: SR-SIM measurement of an LSEC membrane stain obtained on a GE Healthcare DeltaVision|OMX.

From: Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ

Figure 4

Wide-field image (a), Wiener-filtered wide-field (b), single-slice/2D SR-SIM reconstruction by fairSIM (c) and full 3D SR-SIM reconstruction by SoftWORX (manufacturer’s software) (d) are shown for comparison. Both SR-SIM reconstructions allow to clearly identify the cell's fenestrations (tiny membrane pores), which is not possible in the wide-field images. The single-slice reconstructions by fairSIM can be performed with a much lower number of input images, as no z-stack has to be acquired. Scale bar, 5 μm, inset 1.6 μm, cells stained with CellMask Deep Red.

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