Figure 2: The relative expression levels of candidate sp-lncRNAs.

The relative expression of seven candidate sp-lncRNAs of PTEN and PTEN in DU145 and 22RV1 cells was measured by real-time reverse transcription–PCR. The cycle threshold (Ct) difference (ΔCt) between a lncRNA gene and the reference gene RPS28 in the qRT–PCR experiment, which is inversely proportional to the amount of target nucleic acid in the sample, is shown. All experiments were performed in three replicates (n=3). Error bars are defined as s.d.