Figure 5: The sub-cellular localization and the RISC accessibility of lncRNAs. | Nature Communications

Figure 5: The sub-cellular localization and the RISC accessibility of lncRNAs.

From: Integrative analyses reveal a long noncoding RNA-mediated sponge regulatory network in prostate cancer

Figure 5

The RNA level of lnc-2, lnc-6 and lnc-7 in nuclear (Nuc) and cytoplasmic (Cyto) fraction was determined by RT–PCR in (a) DU145 and (b) 22Rv1 cells, respectively. U1 was a positive control for Nuc fraction and GAPDH was a positive control for Cyto fraction. Anti-Ago2-RIP-ChIP for (c) lnc-2, (d) lnc-6 and (e) lnc-7 in DU145 and 22Rv1 cell lines. The relative enrichment with respective to total RNA (input) in both anti-Ago2-RIP and Nonspecific Mouse Serum (NMS) control are shown. All experiments were performed in three biological replicates (n=3). Error bars are defined as s.d. The two-sample t-test was used to calculate the significance of difference between the means of two experimental groups (*P<0.05, **P<0.01, NS: not significant, P⩾0.05).

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