Figure 6: Functional validation of the predicted sp-lncRNAs of PTEN.
(a) Cell proliferation curve of DU145 cells transfected with the siRNA against lnc-2, lnc-6, lnc-7 and PTEN as well as the negative control (Neg Con) siRNA. (b) Cell proliferation curves of the HCT116 Dicer wild-type and HCT116 Dicerex5 cells transfected with the siRNA against lnc-2, lnc-6, lnc-7 and PTEN as well as the Neg Con siRNA. (c) Anchorage-independent growth of DU145 cells transfected with the siRNA against lnc-2, lnc-6, lnc-7 and PTEN as well as the Neg Con siRNA. The bar graph shows the quantification of the colony formation after 10 days. (d) Western blot for phospho-AKT and AKT level following serum starvation and restimulation of DU145 cells transfected with the siRNA against lnc-2, lnc-6, lnc-7 and PTEN as well as the Neg Con siRNA. All experiments with error bars were performed in three replicates (n=3). Error bars are defined as s.d. The two-sample t-test was used to calculate the significance of difference between the means of two experimental groups (*P<0.05, **P<0.01, NS: not significant, P⩾0.05).
