Figure 6: The effects of Gβ2 on mitochondria are specific.
From: G-protein β2 subunit interacts with mitofusin 1 to regulate mitochondrial fusion
(a) Representative images of morphology of endoplasmic reticulum, lysosomes, Golgi, plasma membrane in HeLa cells with enforced expression of Flag-Gβ2. HeLa cells were transfected with Flag-Gβ2. After incubation for 24 h, the cells were fixed in 4% paraformadehyde and double immunostained Flag (green) with either of the antibodies: Calnexin, GM130, Lamp3 or Transferrin receptor (red). (b) HeLa cells were transiently transfected with GFP- Gαi1, GFP-Gαs, Flag-tagged Gβ1or Flag-tagged RACK1, respectively. The cells were then loaded with 20 nM MitoTracker Red for visualizing mitochondria, and immunostained Flag (green) for labelling Flag-tagged Gβ1or Flag-tagged RACK1. (c) Confocal images of HeLa cells transfected with GFP-Gγ2 or Gγ2 (C68S). After incubation for 24 h, the cells were stained with Mitotracker (red). (d) HeLa cells co-transfected Flag-Gβ2 with GFP-Gγ2 or Gγ2 (C68S), and the cell were fixed and immunostained with Flag antibody (red). Sale bar, 10 μm.
