Figure 2: Decreased expression of the miR-378 family in aHSCs. | Nature Communications

Figure 2: Decreased expression of the miR-378 family in aHSCs.

From: MicroRNA-378 limits activation of hepatic stellate cells and liver fibrosis by suppressing Gli3 expression

Figure 2

(a) Expression of miR-378 family members, including miR-378a-3p, miR-378b and miR-378d, in HepG2 (human liver epithelial cell line) and LX2 (human aHSC line) cells was examined by qRT–PCR. (b) qRT–PCR of the miR-378 family in primary qHSCs isolated from normal C57BL/6 mice at quiescent stage (d0; immediately after isolation) and in primary aHSCs (d7; cultured for 7 days). (c) qRT–PCR of the miR-378 family in primary HSCs isolated from corn-oil- (qHSCs) or CCl4-(aHSCs)-treated mice. (d) Expression of the miR-378 family in primary hepatocytes, primary qHSC and LSECs isolated from normal C57BL/6 mice, as assessed by qRT–PCR. All results of relative expression values are shown as mean±s.e.m. of triplicate experiments (unpaired two-sample Student’s t-test, *P<0.05 and **P<0.005).

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