Figure 2: CARD9 in autoantibody-induced skin blistering disease. | Nature Communications

Figure 2: CARD9 in autoantibody-induced skin blistering disease.

From: Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo

Figure 2

Blistering skin disease was triggered in wild type (WT) and Card9−/− intact mice or bone marrow chimeras by systemic injection of collagen VII-specific (anti-CVII) antibodies. Skin disease was followed by photographing on day 14 (a) and clinical assessment of the total body surface affected (b) and the overall disease severity (c). The serum titre of anti-CVII antibodies was tested on day 6 by ELISA (d). Representative images (a) or mean and s.e.m. (b–c) from 7 to 9 control (2 intact and 5–7 bone marrow chimeric) and 13–14 anti-CVII-treated (2 intact and 11–12 bone marrow chimeric) mice per genotype from four independent experiments are shown. No difference between intact and chimeric mice of the same hematopoietic genotype was observed (not shown). (d) Mean and s.e.m. from 4 control and 12–13 anti-CVII-treated mice from five independent experiments are shown. *P<0.05; NS, statistically not significant (two-way ANOVA); see the text for actual P values.

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