Figure 3: CARD9 plays no direct role in myeloid cell migration to the site of inflammation.

Wild type (WT) and Card9^−/− mice were subjected to K/B × N serum-transfer arthritis (a–d), the ankle area or the front paw was flushed on day 4 and the number of neutrophils (a) or monocytes/macrophages (b) was determined by flow cytometry. Mixed bone marrow chimeras with CD45.1-expressing wild type (WT) and CD45.2-expressing WT or Card9^−/− hematopoietic cells were subjected to K/B × N serum-transfer arthritis and in vivo accumulation of neutrophils (c) and monocytes/macrophages (d) was determined by flushing the synovial area on day 4, followed by flow cytometric analysis of the ratio of CD45.1 and CD45.2-expressing cells in peripheral blood and the synovial infiltrate. In (c,d), each dot represents an individual mouse. (e) In vitro migration of WT and Card9^−/− bone marrow neutrophils towards the indicated chemoattractants in a fibrinogen-coated Transwell system. Graphs represent mean and s.e.m. from 5–14 (a) or 6–16 (b) mice per group from five independent experiments. (e) Data represent mean and s.e.m. of three to five independent experiments. *P<0.05; NS, statistically not significant (Student’s t-test (a,b), two-way ANOVA (e)); see the text for actual P values.