Figure 6: CARD9 is required for neutrophil gene expression changes and chemokine/cytokine release. | Nature Communications

Figure 6: CARD9 is required for neutrophil gene expression changes and chemokine/cytokine release.

From: Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo

Figure 6

Wild type (WT) and Card9−/−neutrophils were placed on immobilized immune complex (IC) surfaces. Superoxide release (a) was followed by a spectrophotometric assay, while gelatinase degranulation was determined by gelatinase zymography (b). Cytokine, chemokine and lipid mediator levels in cell-free supernatants were determined after an incubation for 6 h (cytokines) or 1 h (LTB4) using ELISA assays (c,g). Gene expression changes were followed by quantitative PCR (d,h,i) or by Affymetrix Microarrays (e,f). (f) The average changes of the expression of the 50 most highly upregulated genes shown in (e). Kinetic curves in a show mean and s.e.m. of six independent experiments. Control data points were subtracted. (b) Representative of three independent experiments. Graphs (c,d,f–i) show mean and s.e.m. from three to five independent experiments, while the heat map in e represents the colour-coded mean of three independent experiments. *P<0.05; NS, statistically not significant (two-way ANOVA except for h and i where one-way ANOVA was used); see the text for actual P values.

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