Figure 1: SELEX identifies a novel SL RNA ligand of Roquin-1.
From: Roquin recognizes a non-canonical hexaloop structure in the 3′-UTR of Ox40
(a) Enriched hexamers that were found by Roquin-1 N terminus (residues 2–440) or Roquin-1 M199R N terminus (residues 2–440) (see also Supplementary Fig. 1). (b) An ADE sequence motif in the Ox40 3′-UTR closely resembles the MEME motif found in SELEX-enriched RNA sequences. (c) Conservation of the motif found in Ox40 3′-UTRs for various species as indicated. The labels correspond to the versions of the genome assemblies in the UCSC server (see Method section). rn5 is the fifth assembly version of the rat (Rattus novegicus). (d) Schematic representation of the predicted SELEX-derived consensus SL, ADE and the Ox40 ADE-like hexaloop SL. The broken line between the G–G base pair in the ADE SL indicates a putative non-Watson–Crick pairing. The Ox40 CDE-like SL and the Tnf CDE SL are shown for comparison. See also Supplementary Fig. 1.
