Figure 2: NMR analysis of the SL RNAs used in this study.
From: Roquin recognizes a non-canonical hexaloop structure in the 3′-UTR of Ox40
Imino proton regions of one-dimensional 1H NMR spectra of (a) the ADE SL (b), the Ox40 ADE-like SL and (c) the Ox40 CDE-like SL are shown for free RNAs (black) and in complex with the Roquin-1 ROQ domain (red). The respective SL RNAs and their base pairs are indicated. Red asterisks indicate NMR signals of the protein. Black asterisks in a indicate a second conformation (see Supplementary Notes). Green lines in the secondary structure schemes on the left refer to visible imino NMR signals and thus experimental confirmation of the base pairs indicated. Red nucleotides indicate significant chemical shift changes observed. The dotted green line between G6 and G15 in a highlights a G–G base pair.
