Figure 4: NMR analysis of ROQ domain interactions with the Ox40 ADE-like hexaloop RNA.
From: Roquin recognizes a non-canonical hexaloop structure in the 3′-UTR of Ox40
(a) Overlay of 1H,15N HSQC spectra of either the free ROQ domain (171–326, black) or in complex with stoichiometric amounts of the Ox40 ADE-like SL (red). Selected shifts of amide resonances are indicated. (b) Plot of chemical shift change versus residue number in the ROQ domain (residues 171–326) from a. Grey negative bars indicate missing assignments in one of the spectra. Gaps indicate prolines. (c) Overlay of the ROQ domain alone (black) or in complex with the Ox40 ADE-like SL (red) or the Ox40 CDE-like SL (green). See also Supplementary Notes and Supplementary Fig. 3.
