Figure 7: Knockout of the C3aR in APC^Min/+ mice reduces tumour burden and protumorigenic neutrophils in peripheral blood and lymphoid organs.

(a) APC^Min/+ and APC^Min/+/C3aR^−/− mice were killed at the indicated ages and tumours in the small intestine were counted. Data are representative of eight mice per group. (b) Coagulation measured by PT in 12-week-old APC^Min/+ and APC^Min/+/C3aR^−/− mice by using a coagulometer. Data are representative of three independent experiments. Significance was calculated by using unpaired T-test (***P<0.001). (c–g) Single-cell suspensions were prepared from the spleen, BM, mLN and peripheral blood of APC^Min/+ and APC^Min/+/C3aR^−/− mice at the indicated ages, and neutrophils assessed by flow cytometry. Bar graphs show mean number plus s.e.m. in organs or frequencies in blood of CD45.2+ Ly6G+ CD11b+ neutrophils. A minimum of six mice per group was used and results are pooled from two independent experiments. Significance was calculated by using two-way analysis of variance (ANOVA) with Bonferroni post test (NS, not significant; *P<0.05, **P<0.01 and ***P<0.001).