Figure 8: LDNs are prone to produce NETs via a C3aR-dependent mechanism. | Nature Communications

Figure 8: LDNs are prone to produce NETs via a C3aR-dependent mechanism.

From: Coagulation induced by C3aR-dependent NETosis drives protumorigenic neutrophils during small intestinal tumorigenesis

Figure 8

(a–c) Unstimulated HDNs and LDNs purified from 16-week-old APCMin/+, APCMin/+/C3aR−/− and WT mice (only HDNs) were stained with DAPI (4,6-diamidino-2-phenylindole; blue) and MPO (red), and visualized by widefield fluorescence microscopy. In a, representative merged pictures are shown for HDNs and LDNs of APCMin/+ and APCMin/+/C3aR−/− mice. Original magnification, × 20. Scale bar, 40 μm. (b–c) For quantification, NETs were counted on the whole slide and expressed as NET per cm2. Results are pooled from two independent experiments with two to three mice per group. Significance was calculated by using one-way analysis of variance (ANOVA) with Bonferroni post test (*P<0.05). Data in panels b and c is summarized as mean plus s.e.m..

Back to article page