Figure 1: Cellular protein labelling with FP11-tag.
From: Versatile protein tagging in cells with split fluorescent protein
(a) Schematic diagram for FP11-Tag, illustrated on the crystal structure of sfCherry, and the split schemes. (b) Images of HeLa cells co-expressing GFP1-10 and GFP11::β-actin or sfCherry 1-10 and sfCherry 11::β-actin. (c) Average fluorescence intensity of whole cells expressing β-actin labelled with full-length sfGFP, sfCherry or the corresponding FP11 tags. n=6–18. Error bars are s.e.m. (d) Fluorescence images of Drosophila S2 cells expressing GFP11-tagged of β-tubulin and calreticulin, and HeLa cells expressing GFP11-tagged clathrin light chain, histone H2B, zyxin and β2AR. (e) An image of mixed S2 cells expressing either CFP1-10+GFP11::β-actin or YFP1-10+GFP11::β-actin. The weak fluorescence of the CFP1-10-expressing cell in the YFP channel is due to the bleed-through of CFP emission. All the scale bars indicate 5 μm.
