Figure 2: Labelling endogenous proteins using GFP11-tag.
From: Versatile protein tagging in cells with split fluorescent protein
(a) GFP11 knock-in efficiencies by co-transfection with Cas9/sgRNA expression plasmids and donor templates, quantified by the combined TaqMan PCR/ddPCR assay (see Supplementary Fig. 2). (b) GFP fluorescence and immunofluorescence images of knock-in cells. All the error bars are s.e.m. All the scale bars indicate 5 μm. neg, negative.
