Figure 3: Amplification of fluorescence signal by tandem FP11.
From: Versatile protein tagging in cells with split fluorescent protein
(a) Schematic of fluorescence signal amplification by tandem FP11 (b) Images of Drosophila S2 cells expressing GFP11x1, x3 or x7::mCherry::β-tubulin together with overexpressed GFP1-10. The three panel sets were acquired and displayed with identical settings. (c) Quantification of GFP to mCherry fluorescence intensity ratio with different number of GFP11 repeats and different linker lengths between the repeats. Fifty cells were analysed in each case. Error bars are s.e.m. (d) Images of HeLa cells expressing sfCherry11x1 or x4::β-actin together with overexpressed sfCherry1-10. (e) Average whole-cell fluorescence intensity with sfCherry11x1 or x4::β-actin. n as indicated in the figure. Error bars are s.e.m. (f) Two-colour imaging of GFP11-labelled clathrin light chain and sfCherry11x4 labelled β-actin. All the scale bars indicate 5 μm.
