Figure 1: 3D imaging ability of TLS-SPIM in resolving complex structures of multicellular specimens. | Nature Communications

Figure 1: 3D imaging ability of TLS-SPIM in resolving complex structures of multicellular specimens.

From: Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Figure 1

(a–c) 3D volume renderings of a C. elegans embryo (OD95) expressing GFP::PLC∂PH (membrane) and H2B::mCherry (nucleus) at ∼50 cell stage, ∼200 cell stage and bean stage. (d–f) XY lateral slices through the longitudinal axis of the embryo in a, b and c. (g–i) YZ axial slices of the embryo in a-c through the positions marked in d–f. (j–o) XZ axial slices of the embryo in a-c through the positions marked in g–i. (p) 3D volume rendering of a ∼15 h.p.f. zebrafish embryo tailbud expressing KikGR (nucleus). (q–r) A XY lateral slice and a XZ axial slice of the tailbud through the planes in p. Scale bars, 5 μm (d–o) and 20 μm (q–r).

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