Figure 5: 3D imaging by TLS-SPIM with real-time light sheet optimization. | Nature Communications

Figure 5: 3D imaging by TLS-SPIM with real-time light sheet optimization.

From: Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Figure 5

(a) An ideal imaging method with both high spatial resolution and imaging speed acquires spatial and temporal information simultaneously. (b) A compromised solution acquires spatial information and temporal information separately by quick switching between a high spatial resolution, low imaging speed mode and a low spatial resolution, high imaging speed mode. (c,d) 3D volume renderings of two mesodermal cells in the tailbud of a ∼15 hpf zebrafish embryo imaged in a low spatial resolution, high imaging speed mode and a high spatial resolution, low imaging speed mode. (e,f) Orthogonal views of the cells in c and d. (g) Ten continuous time points of the selected area in c imaged in the low spatial resolution, high imaging speed mode, showing the lamellipodia activities of the mesodermal cell. Scale bars, 5 μm (c–f), 2 μm (g).

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