Figure 1: Expression and tonoplast localization of AtPHT5 family members.
From: Identification of plant vacuolar transporters mediating phosphate storage
Expression of p35S:PHT5;1-GFP in Arabidopsis mesophyll protoplasts (a), tobacco (N. benthamiana) leaves (b) and roots of Arabidopsis transgenic plants (c). Expression of pPHT5;1:GUS (d–f), pPHT5;2:GUS (g–i) and pPHT5;3:GUS (j–l) in 12-day-old seedlings grown under Pi-sufficient conditions (d,e,g,h,j,k) and in flowers of transgenic plants grown in soils (f,i,l). Scale bars, 10 μm (a,b,c); 100 μm (d–l). qRT–PCR analysis of PHT5;1 (m) and PHT5;3 (n) expression in the shoot and root of 13-day-old WT seedlings under Pi-sufficient (+) and Pi-deficient (–, 7 days of Pi starvation) conditions. Error bar, s.e. (n=3). **P<0.01, Student’s t-test. Results of qRT–PCR were reproducible in three independent experiments.
