Figure 5: Phosphate transport activity of rice OsSPX-MFS1 in isolated yeast vacuoles.
From: Identification of plant vacuolar transporters mediating phosphate storage
(a) Fluorescence signal of yeast vtc4 mutant transformed with OsSPX-MFS1-ECFP. Arrows indicate the appearance of fluorescence signals in the tonoplast of yeast. Scale bar, 5 μm. 33Pi uptake activity of vacuoles isolated from yeast vtc4 mutants transformed with empty vector (pAG415GPD) or expressing OsSPX-MFS1 in the presence of 4 mM ATP [+ATP] or the absence of ATP [-ATP] at different time points (b) or in the presence of 10 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP) [+CCCP] or the absence of CCCP [−CCCP] at 285 s post incubation (c). Error bar, s.e. (n=3). Results were reproducible in two to four independent experiments.
