Figure 6: Overexpression of PHT5;1 leads to misregulation of PSR genes.

Scatter plots show the comparison of the gene expression (RPKM) in the shoot (a,b) and root (c,d) between the PHT5;1-GFP-overexpressing line #12 and WT plants under Pi-sufficient (+Pi) (a,c) and 3-day Pi-deficient (–Pi) (b,d) conditions. Each dot represents an individual gene entity. Red and green dots represent, respectively, the PSi and PSr genes with differential regulation during Pi starvation in the WT (≥ 2-fold change in the shoot and ≥1.5-fold change in the root). Venn diagrams in each panel indicate the overlaps of the genes differentially expressed in the PHT5;1-GFP-overexpressing line compared with WT and PSR genes of WT. It is noteworthy that the WT PSi genes were upregulated in the PHT5;1-GFP-overexpressing line under +Pi conditions but downregulated under −Pi conditions. In a similar manner, the WT PSr genes were downregulated in PHT5;1-GFP-overexpressing line under +Pi conditions but upregulated under −Pi conditions. (e) Heat map analyses show the expression of a subset of Pi-starvation-upregulated genes with significant gene ontology (GO) enrichment in the category of root development, which were downregulated in the roots of the PHT5;1-GFP-overexpressing line. P-value was adjusted by Bonferroni correction.