Figure 3: Zyxin facilitates Lats2 ubiquitination and degradation.
From: Zyxin-Siah2–Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways
(a) Decreased Lats2 ubiquitylation level by Zyxin-knockdown in vivo. (b) Full-length Zyxin-induced Lats2 ubiquitylation was attenuated by ectopic expression of Siah2RM. (c,d) Zyxin-enhanced Siah2-induced Lats2 degradation (c) and quantification of Myc-Lats2 (normalized to actin) protein levels (d). Ratio t-test was applied. (e,f) Zyxin was required for Siah2-induced Lats2 degradation (e) and quantification of Myc-Lats2 (normalized to actin) protein levels (f). Ratio t-test was applied. All data are mean of n=3 independent experiments. All error bars indicate s.d. *P<0.05; NS as not significant.
