Figure 5: Silencing of circHIPK3 RNA inhibits human cell proliferation. | Nature Communications

Figure 5: Silencing of circHIPK3 RNA inhibits human cell proliferation.

From: Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs

Figure 5

(a) Schematic illustration showing three targeted siRNAs. Si-HIPK3 targets the HIPK3 linear transcript, si-circHIPK3 targets the back-splice junction of circHIPK3, and si-both targets both the linear and circular species. (b) qRT–PCR for circHIPK3 and HIPK3 mRNA in HEK-293 T cells treated with three siRNAs as described above. Data are the means±s.e.m. of three experiments. (c–e) Proliferation of HuH-7, HCT-116 and HeLa cells transfected with the above three siRNAs assessed using a CCK-8 kit at the indicated days. Data in c–e are the means±s.e.m. of three experiments. (f) DNA synthesis assessed using an EdU (5-ethynyl-2’-deoxyuridine) assay in HuH-7 cells transfected with the above three siRNAs for 48 h. Cells were fluorescently stained with EdU (red). Nuclei were stained with DAPI (blue). Micrographs represent at least three experiments. Scale bar, 200 μm. (g) Quantitative EdU assay data from f and Supplementary Fig. 7. Data in d–g are the means±s.e.m. of three experiments. *P<0.05, **P<0.01 (Student’s t-test).

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