Figure 5: Mutations altering LFY oligomerization affect genome-wide LFY DNA binding in planta.
From: A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor
(a) Histogram showing the distribution of CFR values for the LFY-bound regions in p35S:LFY. The inset shows an enlargement for low CFR. (b) AtLFY and AtLFYTERE binding profiles on the AG (left), AP1 (middle) and the TFL1 (right) genomic regions. ChIP-Seq read coverage is shown for p35S:LFY (blue), p35S:LFYTERE (green) and Col-0 (black, background signal). The upper panel shows the LFYbs locations and scores. Blue boxes at the bottom indicate exons. rpb, read per base. (c) Boxplot showing the CFR of the LFY-bound regions with LFYbs of lowest and highest score (first and last deciles). The x axis shows the median LFYbs best score value. P-value for Mann–Whitney rank test is indicated on the graph. (d) Boxplot showing the CFR of the LFY-bound regions with lower and higher LFYbs density (first and last deciles of LFY-bound regions). The x axis shows the median density value (bs kb−1). P-value for Mann–Whitney rank test is indicated on the graph. (e) Heat map representing the distribution of distances between LFYbs. The LFY-bound regions in p35S:LFY were pooled in deciles according to their CFR value (y axis shows the groups median CFR value). The x axis shows the distance (in bp) separating two 19-bp LFYbs. Only distances <40 bp are shown. (f) Boxplot showing the CFR of regions depending on the number of pairs of LFYbs separated by 1 bp (zero, one and two pairs of LFYbs). P-values for Mann–Whitney rank test are indicated on the graph.
