Table 1 Crystallographic data collection and refinement statistics.
From: A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor
GbLFY-N (Native) | GbLFY-N (Se-Met) | |
|---|---|---|
Data collection | ||
Wavelength (Å) | 0.872 | 0.872 |
Resolution (Å) range | 52.34–2.25 (2.33–2.25) | 100–2.61 (2.77–2.61) |
Space group | P 65 | P 65 |
Cell dimensions (Å,°) | a=b=81.1, c=78.5 | a=b=80.6, c=78.5 |
Number of total reflections | 57,705 | 83,685 |
Number of unique reflections | 14,004 | 8,879 |
Multiplicity | 4.1(3.9) | 9.4 (9.2) |
Completeness (%) | 99.3 (99.0) | 99.6 (97.5) |
Mean I/σ(I) | 15.67 (2.89) | 15.28 (2.54) |
R-sym | 0.069 (0.514) | 0.127 (0.915) |
Refinement statistics | ||
Wilson B-factor | 44.1 | 35.85 |
R-factor | 0.1859 (0.2564) | |
R-free | 0.2298 (0.2767) | |
Number of atoms | 1,462 | |
Macromolecule | 1,335 | |
Ligands | 25 | |
Water | 102 | |
Protein residues | 163 | |
Root mean square (bonds) | 0.016 | |
Root mean square (angles) | 1.78 | |
Ramachandran favoured (%) | 99 | |
Ramachandran outliers (%) | 0 | |
Clashscore | 13.40 | |
Average B-factor | 46.80 | |
Macromolecule | 46.00 | |
Solvent | 51.80 | |
