Figure 6: Additional contacts of CgGDE with glycogen.

(a–c) Structures of the additional oligosaccharide-binding sites in GT subdomain B (a), domains M2 (b) and GC (c). (d) Mutations at the additional oligosaccharide-binding sites decrease the affinity to glycogen. CgGDE pulled-down by glycogen immobilized on concanavalin A agarose was analysed by SDS PAGE. (e) Specific debranching activities of CgGDE mutants Y408A, W958A, D1400A and their combinations with mutants possessing only the GT or GC activities.