Figure 1: Apelin/APLNR regulates CXCR4 expression in vitro and in vivo.
From: MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation
(a–d) CXCR4 transcript and protein levels in response to siRNA-mediated knockdown of APLNR (a,b) or APLN (c,d). n=3 experiments per condition. (e) Flow cytometry to determine level of CXCR4 expression in HUVECs transfected with either APLNR-GFP or GFP expression constructs. Data shown represents the CXCR4 staining of cells among the GFP-positive cells. n=3 experiments. (f) Cxcr4 expression (blue) in P5 retinal vessels as assessed by in situ hybridization in Aplnr−/− and Apln−/− mice. Arrows indicate Cxcr4 mRNA localization. Isolectin B4 is shown in white. Scale bar, 100 μm. n=8 retinas per genotype. (g,h) Retinal vasculature of P5 mice with endothelial-specific deletion of Aplnr (Aplnrfl/fl;Cdh5-CreERT2). Graphs depict vascularized area, branchpoints and radial expansion. Scale bar, 200 μm. n≥6 retinas per genotype. (i,j) Effect of AMD3100 on Aplnr−/− retinal vasculature. Scale bar, 200 μm. n≥5 retinas per genotype. *P<0.05, **P≤0.01, ***P≤0.001, t-test. Error bars represent s.e.m.
