Figure 5: Sequence and binding studies of peptide 25.

(a) Sequence of bicyclic peptide 25 showing highest affinity for Rab8a_6–176(GppNHp). (b) Competition of fluorescein-labelled peptide 25 (60 nM) bound to Rab8a(GppNHp; 15 μM) with increasing concentrations of acetylated peptides StRIP3 and wt-R6IP (competitors). Errors represent 1σ of triplicates.