Figure 3: Lack of immature GC spiking using CA recordings.
From: Low excitatory innervation balances high intrinsic excitability of immature dentate neurons
(a, left) Cartoon depicting sequential CA recordings from multiple mature and immature GCs after confirming EPSCs in a WC recording from a mature GC (right). Scale bar, 100 pA, 50 ms. CA recordings were initiated after identifying relatively robust EPSCs in a mature GC (n=9). (b) Examples of spiking in some mature GCs but not in immature GCs. The amplitude and variance of current was not different across cell age and there was no correlation between the noise and spike amplitude (not shown). Scale bar, 20 pA, 20 ms. (c) The fraction of spiking mature GCs was significantly higher than the fraction of spiking immature GCs, both for the CA recordings (left, 22% versus 0%; χ2=4.1) and the combined CA and WC data set (right, 23% versus 0%; χ2=7.29). The total number of recorded cells is indicated in parenthesis.
