Figure 1: An N-terminal portion of FNDC4 is secreted.
From: FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice
(a) Graphical representation of the FNDC4 construct with N-terminal FLAG and C-terminal Myc tags as well as the peptide sequence. C, intracellular domain; FN III domain, Fibronectin type III domain; H, hydrophobic domain; SP, signal peptide. (b) Western blot against either FLAG or Myc, as indicated, in cell- and media samples from HEK293 cells transfected with the N-terminal FLAG and C-terminal Myc FNDC4 construct. The full-size blot image is presented in Supplementary Fig. 11a. Similar results were seen in at least three independent experiments. NT, non-transfected control.
