Figure 2: Fndc4 gene expression is upregulated in inflammatory conditions.
From: FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice
(a) Fndc4 gene expression in the murine colon on induction of DSS-mediated colitis. DSS was administered via the drinking water, mice were sacrificed 7 days later, N=10 mice per group. NT, non-treated. Mean±s.e.m., *P<0.05 (Student’s T-test). This experiment was performed three times with similar results. (b) Fndc4 gene expression in the kidney on induction of glomerulonephritis. Mice were injected with antiserum directed against the glomerular basement membrane (αGBM) and sacrificed 5 days post injection. NT, non-treated. N=5 mice per group. Mean±s.e.m., *P<0.05 (Student’s T-test). This experiment was repeated once with similar results. (c–d) Human IBD cohort. FNDC4 gene expression in intestinal biopsies from inflamed versus non-inflamed regions of the same patients with ulcerative colitis (UC) (N=12) (c) or Crohn’s disease (CD) (N=7) (d). *P<0.05, paired T-tests. (e) RNA in situ hybridization. Distal colon from DSS-treated mice. Fndc4 versus negative control (NC) probe. Arrows point at examples of positive signals (red).
