Figure 3: TGFβ induces FNDC4 expression.
From: FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice
(a) FNDC4 expression in Caco-2 cells after 20 h treatment with 20 ng ml−1 TGFβ1. N=4. Mean±s.e.m., *P<0.05, Student’s T-test. (b) Luciferase assay. McA-RH7777 cells were transfected with an Fndc4 promoter reporter construct and treated with 20 ng ml−1 TGFβ1 and/or TGFβ1R antagonist SB-431542 (SB) (20 μM) for 20 h. N=4–5. Mean±s.e.m., *P<0.05 (one-way analysis of variance with Tukey’s post hoc tests).
