Figure 5: FNDC4 acts on macrophages.
From: FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice
(a) Immune cell infiltration of DSS-mediated inflamed colon mostly consists of macrophages. Flow cytometry analysis of CD11b+-cells amongst CD45+ cells in the DSS colitis model. N=10 for the hFc control group, N=9 for the hFc-FNDC4 group. (b) FNDC4 binds to IgG Fc-gamma (IgG Fc-receptor negative) macrophages. The plots on the left show binding to peritoneal macrophages, the graph on the right shows binding to a panel of blood cells. Antibodies against CD11b and F4/80 were used to gate for macrophages. Binding of hFc-FNDC4 to cells was detected using a PE-conjugated antibody against human IgG. (c) ERK phosphorylation after 30 minutes of hFc-FNDC4 treatment. Total ERK served as loading control. This experiment was repeated twice with similar results. The full-size blot image is presented in Supplementary Fig. 11b. (d) Dose curve of hFc-FNDC4-mediated induction of Ass1 and Myo7a in bone marrow macrophages treated for 24 h with different concentrations of hFc-FNDC4 (100 nM hFc as control). Data are presented as mean±s.e.m., N=4. *P<0.05, One-way analysis of variance followed by Dunnett’s post hoc tests.
