Figure 1: Primary screen for CHIKV host cell factors.
From: A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs
(a) Outline of screening procedure. (b) Heatmap of all identified proviral and antiviral hits showing replication data (Z-scores) of the four most efficient siRNAs. Arrowheads indicate genes experimentally characterized in this study. (c) Requirement of identified proviral factors for other viruses based on published loss-of-function studies. Viruses other than CHIKV are indicated by specific colours, and colour-coded boxes contain genes shared between CHIKV and the corresponding virus. White boxes contain genes shared between CHIKV and more than one other virus. Full details in Supplementary Data 3. (d) Heatmap illustrating the replication capability of CHIKV in Cas9-positive HEK-293T cells expressing the indicated gRNAs. Cells were infected with CHIK-GFP at MOI 6 for 24 h (n=9). (e) Validation of CLK1 as CHIKV relevant host factor. Infection rate of A549 cells depleted for CLK1 by CRISPR/Cas9 (see Supplementary Fig. 1j for more details) and infected with CHIKV-GFP for the indicated periods of time (n=9 for each data set). Data represent the means±s.e.m. of three independent experiments and were analysed using one-way analysis of variance with Tukey’s post test. (*P<0.05; NSP≥0.05). CTRL, control; NS, not significant.
